PHOTOSENSITIVE NITRILE HYDRATASE INTRINSICALLY POSSESSES NITRIC-OXIDE BOUND TO THE NONHEME IRON CENTER - EVIDENCE BY FOURIER-TRANSFORM INFRARED-SPECTROSCOPY

被引：88

作者：

NOGUCHI, T

HONDA, J

NAGAMUNE, T

SASABE, H

INOUE, Y

ENDO, I

机构：

[1] RIKEN,FRONTIER RES PROGRAM,WAKO,SAITAMA 35101,JAPAN

[2] UNIV TOKYO,FAC ENGN,BUNKYO KU,TOKYO 113,JAPAN

[3] RIKEN,CHEM ENGN LAB,WAKO,SAITAMA 35101,JAPAN

来源：

FEBS LETTERS | 1995年 / 358卷 / 01期

关键词：

NITRIC OXIDE; NITRILE HYDRATASE; FOURIER TRANSFORM INFRARED SPECTROSCOPY; NONHEME IRON; PHOTOACTIVATION;

D O I：

10.1016/0014-5793(94)01374-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitrile hydratase (NHase) from Rhodococcus sp, N-771 is a photosensitive enzyme that catalyzes hydration of nitriles to the corresponding amides. Light-induced Fourier transform infrared difference spectra between the inactive and active forms of NHase were measured with both the natural (N-14) and N-15-labeled NHases. The results showed, for the first time, that NHase intrinsically possesses nitric oxide (NO) molecules bound to the non-heme iron center. The possible role of NO in the photoactivation process of NHase is discussed.

引用

页码：9 / 12

页数：4

共 30 条

[1] A BACTERIAL, NITRIC-OXIDE SYNTHASE FROM A NOCARDIA SPECIES [J].