FUNCTIONAL-CHARACTERISTICS OF 2 D-XYLANASES PURIFIED FROM TRICHODERMA-HARZIANUM

被引：29

作者：

TAN, LUL

WONG, KKY

SADDLER, JN

机构：

[1] Biotechnology and Chemistry Department, Forintek Canada Corporation, Ottawa, K1G 3Z5, Canada

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1985年 / 7卷 / 09期

关键词：

BACTERIOLOGY; -; BIOMASS;

D O I：

10.1016/0141-0229(85)90042-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two endo-1,4- beta -D-xylanases were purified from Trichoderma harzianum culture filtrates. From kinetic analyses, apparent V//m//a//x and K//m values of 580 U mg** minus **1 protein and 0. 16% D-xylan were obtained for the 20,000 dalton endo-1,4- beta -D-xylanase, while values of 100 U mg** minus **1 protein and 0. 066% D-xylan were obtained for the 29,000 dalton endo-1,4- beta -D-xylanase. Substrate levels greater than 1% (w/ nu ) D-xylan were found to be inhibitory to both enzymes. Both D-xylanases were highly active against D-xylans obtained from various sources. Of the polymeric sugars tested, carboxymethyl cellulose was the only substrate which was hydrolyzed to any extent.

引用

页码：431 / 436

页数：6

共 19 条

[1] XYLAN-DEGRADING ENZYMES OF THE YEAST CRYPTOCOCCUS-ALBIDUS - IDENTIFICATION AND CELLULAR-LOCALIZATION [J].