ELECTRICAL-ACTIVITY CONTROLLING SYSTEM FOR A MEDIATOR-COEXISTING ALCOHOL-DEHYDROGENASE NAD CONDUCTIVE MEMBRANE

被引：79

作者：

YABUKI, S ^{[1
]}

SHINOHARA, H ^{[1
]}

IKARIYAMA, Y ^{[1
]}

AIZAWA, M ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,DEPT BIOENGN,O-OKAYAMA 2-12-1,MEGURO KU,TOKYO 152,JAPAN

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1990年 / 277卷 / 1-2期

关键词：

D O I：

10.1016/0022-0728(90)85100-J

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A mediator-coexisting alcohol dehydrogenase-NAD conductive membrane was prepared by electro-chemical polymerization in a pyrrole solution containing alcohol dehydrogenase, NAD, and Meldola's blue. The polypyrrole membrane has enzyme activity as well as electroconductivity. NADH, produced enzymatically in the presence of ethyl alcohol, was oxidized with the aid of Meldola's blue in the membrane. The current of NADH oxidation was dependent on the alcohol concentration as well as the applied potential, i.e., the enzyme activity of the membrane was controlled electrically by the smooth and efficient electron transferring system, ethanol-enzyme-coenzyme-mediator, prepared in a polypyrrole matrix as a conductive interface. © 1990.

引用

页码：179 / 187

页数：9

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