ELEVATED LEVELS OF THROMBIN-HEPARIN COFACTOR-II COMPLEX IN PLASMA FROM PATIENTS WITH DISSEMINATED INTRAVASCULAR COAGULATION

被引:7
作者
ANDERSSON, TR
SIE, P
PELZER, H
AAMODT, LM
NUSTAD, K
ABILDGAARD, U
机构
[1] CTR TRANSFUS SANGUINE,HEMOSTASE LAB,TOULOUSE,FRANCE
[2] RADIUMHOSP,CENT LAB,N-0310 OSLO,NORWAY
[3] BEHRINGWERKE AG,DEPT BLOOD COAGULAT,W-3550 MARBURG,GERMANY
关键词
THROMBIN; HEPARIN COFACTOR-II; DISSEMINATED INTRAVASCULAR COAGULATION; ELISA ASSAY; PROTEASE-INHIBITOR COMPLEX;
D O I
10.1016/0049-3848(92)90313-Y
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
An ELISA assay for quantitation of the thrombin-heparin cofactor II complex (T-HC II) in plasma was developed. Plasma was incubated with immobilized, specific antibodies to human thrombin. The second, biotinylated antibody was directed against human HC II. The assay was insensitive to thrombin-antithrombin complex (TAT) and to uncomplexed HC II. In plasma samples from 31 normal individuals (aged 21 - 68, mean 43.3 years), the T-HC II ranged 0.3 - 6.1 ng/ml; median 1.5, mean 2.0, and SD 1.6 ng/ml. In plasma samples from 13 patients with disseminated intravascular coagulation (DIC), T-HC II ranged 0.4 - 30.0 (median 13.5) ng/ml. In plasma samples from 6 patients in which the clinical suspicion of DIC was not verified, T-HC II complex ranged 1.4 - 14.3 (median 3.6) ng/ml. In plasma samples with elevated T-HC II levels, TAT was usually elevated, and on the average more than was T-HC II. These results indicate that HC II contributes significantly to the inactivation of in vivo generated thrombin.
引用
收藏
页码:591 / 598
页数:8
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