INTERACTIONS OF PORPHYROMONAS-GINGIVALIS WITH EPITHELIAL-CELLS

被引：159

作者：

DUNCAN, MJ

NAKAO, S

SKOBE, Z

XIE, H

机构：

来源：

INFECTION AND IMMUNITY | 1993年 / 61卷 / 05期

关键词：

D O I：

10.1128/IAI.61.5.2260-2265.1993

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The invasion of gingival epithelial cells by certain pathogenic periodontal bacteria may account for their presence within diseased gingival tissue. To dissect the initial steps of a potential invasion pathway for the periodontal pathogen Porphyromonas gingivalis, laboratory and clinical bacterial isolates were tested for their interactions with a human oral epithelial cell line (KB). Several P. gingivalis strains immobilized on filters could bind oral epithelial cells. Quantitative adherence assays supported these results. The invasion of epithelial cells by P. gingivalis 33277 was measured by assay and confirmed by transmission electron microscopy. These preliminary results demonstrate that certain P. gingivalis strains are capable of internalization by human oral epithelial cells in vitro.

引用

收藏

页码：2260 / 2265

页数：6

相关论文

共 17 条

[1] SELECTIVE MODULATION OF BACTERIAL ATTACHMENT TO ORAL EPITHELIAL-CELLS BY ENZYME-ACTIVITIES ASSOCIATED WITH POOR ORAL HYGIENE [J].

CHILDS, WC ;

GIBBONS, RJ .

JOURNAL OF PERIODONTAL RESEARCH, 1990, 25 (03) :172-178

[2] TISSUE LOCALIZATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS IN HUMAN PERIODONTITIS .1. LIGHT, IMMUNOFLUORESCENCE AND ELECTRON-MICROSCOPIC STUDIES [J].

CHRISTERSSON, LA ;

ALBINI, B ;

ZAMBON, JJ ;

WIKESJO, UME ;

GENCO, RJ .

JOURNAL OF PERIODONTOLOGY, 1987, 58 (08) :529-539

[3] HELA-CELL INFECTION BY YERSINIA-ENTEROCOLITICA - EVIDENCE FOR LACK OF INTRACELLULAR MULTIPLICATION AND DEVELOPMENT OF A NEW PROCEDURE FOR QUANTITATIVE EXPRESSION OF INFECTIVITY [J].

DEVENISH, JA ;

SCHIEMANN, DA .

INFECTION AND IMMUNITY, 1981, 32 (01) :48-55

[4]

DUNCAN MA, UNPUB

[5] DISCRIMINATION BETWEEN INTRACELLULAR UPTAKE AND SURFACE-ADHESION OF BACTERIAL PATHOGENS [J].

ISBERG, RR .

SCIENCE, 1991, 252 (5008) :934-938

[6] A SINGLE GENETIC-LOCUS ENCODED BY YERSINIA-PSEUDOTUBERCULOSIS PERMITS INVASION OF CULTURED ANIMAL-CELLS BY ESCHERICHIA-COLI K-12 [J].

ISBERG, RR ;

FALKOW, S .

NATURE, 1985, 317 (6034) :262-264

[7] SPECIFIC-INHIBITION OF ADHERENCE OF AN ORAL-STRAIN OF BACTEROIDES-GINGIVALIS 381 TO EPITHELIAL-CELLS BY MONOCLONAL-ANTIBODIES AGAINST THE BACTERIAL FIMBRIAE [J].

ISOGAI, H ;

ISOGAI, E ;

YOSHIMURA, F ;

SUZUKI, T ;

KAGOTA, W ;

TAKANO, K .

ARCHIVES OF ORAL BIOLOGY, 1988, 33 (07) :479-485

[8] COMPARATIVE-STUDIES OF THE OUTER MEMBRANES OF BACTEROIDES-GINGIVALIS, STRAINS ATCC-33277, W50, W83, 381 [J].

KENNELL, W ;

HOLT, SC .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 1990, 5 (03) :121-130

[9] BINDING OF CULTURED-MAMMALIAN-CELLS TO IMMOBILIZED BACTERIA [J].

LEONG, JM ;

DEVARGAS, LM ;

ISBERG, RR .

INFECTION AND IMMUNITY, 1992, 60 (02) :683-686

[10] EVIDENCE FOR INVASION OF A HUMAN ORAL CELL-LINE BY ACTINOBACILLUS-ACTINOMYCETEMCOMITANS [J].

MEYER, DH ;

SREENIVASAN, PK ;

FIVESTAYLOR, PM .

INFECTION AND IMMUNITY, 1991, 59 (08) :2719-2726