THE RELATIVE IMPORTANCE OF ESCHERICHIA-COLI EXONUCLEASE-III AND ENDONUCLEASE-IV FOR THE HYDROLYSIS OF 3'-PHOSPHOGLYCOLATE ENDS IN POLYDEOXYNUCLEOTIDES

被引：21

作者：

SIWEK, B ^{[1
]}

BRICTEUXGREGOIRE, S ^{[1
]}

BAILLY, V ^{[1
]}

VERLY, WG ^{[1
]}

机构：

[1] UNIV LIEGE, FAC SCI, SART TILMAN B6, B-4000 LIEGE 1, BELGIUM

来源：

NUCLEIC ACIDS RESEARCH | 1988年 / 16卷 / 11期

关键词：

D O I：

10.1093/nar/16.11.5031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro, in the presence of Mg++, the 3''-phosphoglycolatase activity of endonuclease IV is about 4-times smaller than that of exonuclease III for the same AP endonuclease activity. It thus seems that endonuclease IV has only a minor role in the repair of strand breaks limited by 3''-phosphoglycolate ends in Escherichia coli even after the amount of enzyme has been increased by induction with O2-generating agents.

引用

页码：5031 / 5038

页数：8

共 11 条

[1] ENDONUCLEASE-IV OF ESCHERICHIA-COLI IS INDUCED BY PARAQUAT [J].

CHAN, E ;

WEISS, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (10) :3189-3193

[2] EXONUCLEASE-III AND ENDONUCLEASE-IV REMOVE 3' BLOCKS FROM DNA-SYNTHESIS PRIMERS IN H2O2-DAMAGED ESCHERICHIA-COLI [J].

DEMPLE, B ;

JOHNSON, A ;

FUNG, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (20) :7731-7735

[3] PROPERTIES OF MAIN ENDONUCLEASE SPECIFIC FOR APURINIC SITES OF ESCHERICHIA-COLI (ENDONUCLEASE-VI) - MECHANISM OF APURINIC SITE EXCISION FROM DNA [J].

GOSSARD, F ;

VERLY, WG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 82 (02) :321-332

[4]

HENNER WD, 1983, J BIOL CHEM, V258, P711

[5]

HENNER WD, 1983, J BIOL CHEM, V258, P5198

[6] METHYL METHANE SULFONATE-SENSITIVE MUTANT OF ESCHERICHIA-COLI DEFICIENT IN AN ENDONUCLEASE SPECIFIC FOR APURINIC SITES IN DEOXYRIBONUCLEIC-ACID [J].

LJUNGQUIST, S ;

LINDAHL, T ;

HOWARDFLANDERS, P .

JOURNAL OF BACTERIOLOGY, 1976, 126 (02) :646-653

[7]

LJUNGQUIST S, 1977, J BIOL CHEM, V252, P2808

[8] REPAIR OF X-RAY-INDUCED DEOXYRIBONUCLEIC-ACID SINGLE-STRAND BREAKS IN XTH MUTANTS OF ESCHERICHIA-COLI [J].

SEEBERG, E ;

STEINUM, AL .

JOURNAL OF BACTERIOLOGY, 1980, 141 (03) :1424-1427

[9] ENDONUCLEASE FOR DEPURINATED DNA IN ESCHERICHIA-COLI B [J].

VERLY, WG ;

PAQUETTE, Y .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1972, 50 (02) :217-&

[10]

WEISS B, 1976, J BIOL CHEM, V251, P1896

← 1 2 →