SEPARATION OF PRISTINAMYCINS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY .1. SELECTION OF SOLVENT SYSTEM AND PRELIMINARY PREPARATIVE STUDIES

被引：13

作者：

DROGUE, S ^{[1
]}

ROLET, MC ^{[1
]}

THIEBAUT, D ^{[1
]}

ROSSET, R ^{[1
]}

机构：

[1] ECOLE SUPER PHYS & CHIM IND,CHIM ANALYT LAB,10 RUE VAUQUELIN,F-75005 PARIS,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 593卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80305-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Partition coefficient determination and direct application of high-speed counter-current chromatography allowed the selection of suitable chloroform-ethyl acetate-methanol-water solvent systems for the separation of macrolide antibiotics. Sample loading limits were found to be 10 ml and 200 mg for a total volume capacity of 108 ml. This allows scale-up to higher volume counter-current chromatographic apparatus and direct competition with semi-preparative high-performance liquid chromatography for crude extract samples.

引用

页码：363 / 371

页数：9

共 26 条

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