EXPRESSION OF BACTERIAL CYTOTOXIN GENES IN MAMMALIAN TARGET-CELLS

被引：12

作者：

WELS, W ^{[1
]}

BALDRICH, M ^{[1
]}

CHAKRABORTY, T ^{[1
]}

GROSS, R ^{[1
]}

GOEBEL, W ^{[1
]}

机构：

[1] UNIV WURZBURG,BIOZENTRUM,THEODOR BOVERI INST BIOWISSENSCH,LEHRSTUHL MIKROBIOL,W-8700 WURZBURG,GERMANY

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 18期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01442.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the expression of the gene fragments encoding the enzymatically active portion of three bacterial cytotoxins: exotoxin A (ETA) of Pseudomonas aeruginosa, and pertussis toxin (PT) and adenylate cyclase toxin (CYA) of Bordetella pertussis, in sensitive mammalian target cells. Expression of active ETA and CYA was lethal to the producing cells and stable transfectants of Cos-1 cells containing the corresponding genes could not be obtained. The expression of the PTS1 subunit was tolerated by the producing mammalian cells. Since PT is cytotoxic because of ADP-ribosylation of G-proteins, we assume that the endogenously expressed PTS1 may not find the cellular target G proteins or PTS1 alone may not be sufficient for ADP-ribosylation of these proteins in vivo.

引用

页码：2651 / 2659

页数：9

共 32 条

[1] ADP-RIBOSYLTRANSFERASE MUTATIONS IN THE CATALYTIC S-1 SUBUNIT OF PERTUSSIS TOXIN [J].