EVIDENCE FOR THE ROLE OF SOLVATED METAL HYDROXIDE IN THE HAMMERHEAD CLEAVAGE MECHANISM

被引：312

作者：

DAHM, SC ^{[1
]}

DERRICK, WB ^{[1
]}

UHLENBECK, OC ^{[1
]}

机构：

[1] UNIV COLORADO, DEPT CHEM & BIOCHEM, BOULDER, CO 80309 USA

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 48期

关键词：

D O I：

10.1021/bi00211a013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The log of the rate of the chemical step of hammerhead cleavage in Mg2+ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates that a single deprotonation is required for cleavage. Hammerhead pH-rate profiles with Ca2+, Mn2+, Co2+, and Cd2+ correlate well with the pK(a)'s of these ions in water. This relationship between the pK(a)'s and the pH-rate profile suggests that a metal hydroxide bound to the hammerhead RNA acts as the base in the cleavage mechanism.

引用

页码：13040 / 13045

页数：6

共 57 条

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