PRODUCTION OF 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID USING SOYBEAN LIPOXYGENASE-1 IN A BIPHASIC OCTANE-WATER SYSTEM

被引：28

作者：

DROUET, P

THOMAS, D

LEGOY, MD

机构：

[1] Laboratoire de Technologie Enzymatique. Université de Technologie de Compiègne., 60206 Compiègne

来源：

TETRAHEDRON LETTERS | 1994年 / 35卷 / 23期

关键词：

D O I：

10.1016/S0040-4039(00)76703-7

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The synthetic potential of soybean lipoxygenase 1 (LOX 1) for the synthesis of 13(S) hydroperoxy-9(Z),11(E)-octadecadienoic acid is investigated in a biphasic medium (octane; berate buffer, pH 9.6) Improvement of the reaction yield (compared to an aqueous system) is observed at very high concentrations (20-40 g/L). The regioselectivity of the reaction is not affected by the presence of the organic phase.

引用

收藏

页码：3923 / 3926

页数：4

相关论文

共 11 条

[1] STEADY-STATE KINETICS OF OXYGENATION OF LINOLEIC-ACID CATALYZED BY SOYBEAN LIPOXYGENASE [J].

EGMOND, MR ;

BRUNORI, M ;

FASELLA, PM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 61 (01) :93-100

[2]

Grossman S, 1979, Methods Biochem Anal, V25, P303, DOI 10.1002/9780470110454.ch5

[3] PRODUCTION OF 9-HYDROPEROXY-GAMMA-LINOLENIC ACID BY SOYBEAN LIPOXYGENASE IN A 2-PHASE SYSTEM [J].

HIRUTA, O ;

NAKAHARA, T ;

YOKOCHI, T ;

KAMISAKA, Y ;

SUZUKI, O .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1988, 65 (12) :1911-1914

[4]

ICAZIO G, 1990, J ORG CHEM, V55, P1690

[5] LIPOXYGENASE CATALYZED HYDROPEROXIDE FORMATION IN MICROEMULSIONS CONTAINING NONIONIC SURFACTANT [J].

PIAZZA, GJ .

BIOTECHNOLOGY LETTERS, 1992, 14 (12) :1153-1158

[6] EFFECT OF LIPID HYDROPEROXIDE ON LIPOXYGENASE KINETICS [J].

SCHILSTRA, MJ ;

VELDINK, GA ;

VERHAGEN, J ;

VLIEGENTHART, JFG .

BIOCHEMISTRY, 1992, 31 (33) :7692-7699

[7] DOUBLE DIOXYGENATION OF ARACHIDONIC-ACID BY SOYBEAN LIPOXYGENASE-1 - KINETICS AND REGIO-STEREO SPECIFICITIES OF THE REACTION STEPS [J].

VANOS, CPA ;

RIJKESCHILDER, GPM ;

VANHALBEEK, H ;

VERHAGEN, J ;

VLIEGENTHART, JFG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 663 (01) :177-193

[8]

Veldink G A, 1984, Adv Inorg Biochem, V6, P139

[9] MICELLE AND ACID-SOAP FORMATION OF LINOLEIC-ACID AND 13-L-HYDROPEROXYLINOLEIC ACID BEING SUBSTRATES OF LIPOXYGENASE-1 [J].

VERHAGEN, J ;

VLIEGENTHART, JFG ;

BOLDINGH, J .

CHEMISTRY AND PHYSICS OF LIPIDS, 1978, 22 (04) :255-259

[10]

VIGNOLO R, 1991, INFORM, V2, P692