PHYSICAL AND GENETIC-MAP OF THE CHROMOSOME OF THE UNICELLULAR CYANOBACTERIUM SYNECHOCYSTIS SP STRAIN PCC-6803

被引:15
作者
CHURIN, YN
SHALAK, IN
BORNER, T
SHESTAKOV, SV
机构
[1] HUMBOLDT UNIV BERLIN,DEPT GENET,D-10115 BERLIN,GERMANY
[2] MOSCOW MV LOMONOSOV STATE UNIV,DEPT GENET,MOSCOW 119899,RUSSIA
关键词
D O I
10.1128/jb.177.11.3337-3343.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A combined physical and genetic map of the cyanobacterium Synechocystis sp. strain PCC 6803 chromosome was constructed. An estimated genome size of 3.82 Mb was obtained by summing the sizes of 25 MluI or 40 NotI fragments seen by pulsed-field electrophoresis. The order of the restriction fragments was determined by using two independent experimental approaches: pulsed-field fragment hybridization and linking clone analysis. The relative positions of 30 known genes or gene clusters were localized.
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页码:3337 / 3343
页数:7
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