SEQUENTIAL CENTRIFUGAL LAYER CHROMATOGRAPHY (SCLC) - A NEW TECHNIQUE FOR THE ISOLATION OF NATURAL COMPOUNDS .2. COMPARATIVE-STUDY ON CENTRIFUGAL LAYER CHROMATOGRAPHY (CLC) AND SCLC FOR THE SEPARATION OF FUROCOUMARIN ISOMERS

被引：9

作者：

ERDELMEIER, CAJ ^{[1
]}

NYIREDY, S ^{[1
]}

STICHER, O ^{[1
]}

机构：

[1] ETH ZURICH, INST PHARMAZEUT, CH-8092 ZURICH, SWITZERLAND

来源：

JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS | 1985年 / 8卷 / 03期

关键词：

D O I：

10.1002/jhrc.1240080305

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The new sequential centrifugal layer chromatography extends the possibilities for preparative separation of furocoumarin isomers. In the present work, isobergapten, pimpinellin, bergapten, isopimpinellin and sphondin were isolated from a prepurified extract of Heracleum sphondylium roots using 2 different techniques, namely, centrifugal layer chromatography (CLC) and sequential centrifugal layer chromatography (SCLC). The former technique was carried out with 4 plates, whereas the latter method required only 1 plate, using the same mobile phase in each case. A further advantage of the SCLC method was that all completely separated compounds could be quickly and efficiently eluted in small volumes of methanol.

引用

页码：132 / 134

页数：3