PURIFICATION AND CRYSTALLIZATION OF THE CATALYTIC DOMAIN OF HUMAN PROTEIN-TYROSINE-PHOSPHATASE 1B EXPRESSED IN ESCHERICHIA-COLI

被引：45

作者：

BARFORD, D

KELLER, JC

FLINT, AJ

TONKS, NK

机构：

[1] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724

[2] WM KECK STRUCT BIOL LAB,COLD SPRING HARBOR,NY 11724

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 239卷 / 05期

关键词：

PROTEIN TYROSINE PHOSPHATASES; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; PROTEIN CRYSTALLOGRAPHY;

D O I：

10.1006/jmbi.1994.1409

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The amino-terminal 321 residues encoding the catalytic domain of human protein tyrosine phosphatase 1B (molecular mass 37 kDa) has been expressed in Escherichia coli, purified to homogeneity and crystallized. The crystals diffract to 2.4 Å resolution when exposed to synchrotron radiation and belong to space group P3121 (or its enantiomorph P3221) with α = 88.4 Å, b = 88.4 Å, c = 104.0 Å, α = β = 90.0°, γ = 120.0°. There is one molecule of protein tyrosine phosphatase 1B per asymmetric unit and the crystal form is suitable for the determination of the atomic structure of the enzyme. © 1994 Academic Press Limited.

引用

页码：726 / 730

页数：5

共 38 条

[1] ALESSI DR, 1993, ONCOGENE, V8, P2015
[2] BROWNSHIMER S, 1992, CANCER RES, V52, P478
[3] MOLECULAR-CLONING AND CHROMOSOME MAPPING OF THE HUMAN GENE ENCODING PROTEIN PHOSPHOTYROSYL PHOSPHATASE-1B
BROWNSHIMER, S
JOHNSON, KA
LAWRENCE, JB
JOHNSON, C
BRUSKIN, A
GREEN, NR
HILL, DE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (13) : 5148 - 5152
[4] HUMAN-PLACENTA PROTEIN-TYROSINE-PHOSPHATASE - AMINO-ACID SEQUENCE AND RELATIONSHIP TO A FAMILY OF RECEPTOR-LIKE PROTEINS
CHARBONNEAU, H
TONKS, NK
KUMAR, S
DILTZ, CD
HARRYLOCK, M
COOL, DE
KREBS, EG
FISCHER, EH
WALSH, KA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) : 5252 - 5256
[5] 1002 PROTEIN PHOSPHATASES
CHARBONNEAU, H
TONKS, NK
[J]. ANNUAL REVIEW OF CELL BIOLOGY, 1992, 8 : 463 - 493
[6] CLONING OF A CDNA FOR A MAJOR HUMAN PROTEIN-TYROSINE-PHOSPHATASE
CHERNOFF, J
SCHIEVELLA, AR
JOST, CA
ERIKSON, RL
NEEL, BG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) : 2735 - 2739
[7] ISOLATION AND STRUCTURAL ELUCIDATION OF A NOVEL PHOSPHOCYSTEINE INTERMEDIATE IN THE LAR PROTEIN TYROSINE PHOSPHATASE ENZYMATIC PATHWAY
CHO, HJ
KRISHNARAJ, R
KITAS, E
BANNWARTH, W
WALSH, CT
ANDERSON, KS
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (18) : 7296 - 7298
[8] SIGNALING BY RECEPTOR TYROSINE KINASES
FANTL, WJ
JOHNSON, DE
WILLIAMS, LT
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1993, 62 : 453 - 481
[9] MULTISITE PHOSPHORYLATION OF THE PROTEIN-TYROSINE PHOSPHATASE, PTP1B - IDENTIFICATION OF CELL-CYCLE REGULATED AND PHORBOL ESTER STIMULATED SITES OF PHOSPHORYLATION
FLINT, AJ
GEBBINK, MFGB
FRANZA, BR
HILL, DE
TONKS, NK
[J]. EMBO JOURNAL, 1993, 12 (05) : 1937 - 1946
[10] THE NONTRANSMEMBRANE TYROSINE PHOSPHATASE PTP-1B LOCALIZES TO THE ENDOPLASMIC-RETICULUM VIA ITS 35 AMINO-ACID C-TERMINAL SEQUENCE
FRANGIONI, JV
BEAHM, PH
SHIFRIN, V
JOST, CA
NEEL, BG
[J]. CELL, 1992, 68 (03) : 545 - 560

← 1 2 3 4 →