PRODUCTION AND PROPERTIES OF A FACTOR-X CELLULOSE-BINDING DOMAIN FUSION PROTEIN

被引：19

作者：

ASSOULINE, Z ^{[1
]}

SHEN, H ^{[1
]}

KILBURN, DG ^{[1
]}

WARREN, RAJ ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA, DEPT MICROBIOL, 300-6174 UNIV BLVD, VANCOUVER V6T 1Z3, BC, CANADA

来源：

PROTEIN ENGINEERING | 1993年 / 6卷 / 07期

基金：

英国医学研究理事会;

关键词：

FACTOR-X; CELLULOSE-BINDING DOMAIN; FUSION PROTEIN; CELLULOSE; AFFINITY CHROMATOGRAPHY; IMMOBILIZED ENZYME;

D O I：

10.1093/protein/6.7.787

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fusion protein, FX-CBD(Cex), which comprises factor X with a cellulose-binding domain (CBD(Cex)) fused to its C-terminus, was produced in BHK cells. It was purified from the culture medium by affinity chromatography on cellulose. FX-CBD(Cex) could be activated to FXa-CBD(Cex) with Russell viper venom. FXa-CBD(Cex) was as active as FXa against a chromogenic substrate and against proteins containing the Ile - Glu - Gly - Arg sequence hydrolysed by FXa. FXa-CBD(Cex) retained its activity when adsorbed to cellulose.

引用

页码：787 / 792

页数：6

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