DETECTION OF FELINE CORONAVIRUS RNA IN FECES, TISSUES, AND BODY-FLUIDS OF NATURALLY INFECTED CATS BY REVERSE-TRANSCRIPTASE PCR

被引:175
作者
HERREWEGH, AAPM [1 ]
DEGROOT, RJ [1 ]
CEPICA, A [1 ]
EGBERINK, HF [1 ]
HORZINEK, MC [1 ]
ROTTIER, PJM [1 ]
机构
[1] UNIV PRINCE EDWARD ISL,ATLANTIC VET COLL,DEPT PATHOL & MICROBIOL,CHARLOTTETOWN,PE C1A 4P3,CANADA
关键词
D O I
10.1128/JCM.33.3.684-689.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A nested reverse transcriptase PCR (RT-nPCR) was developed for the detection of feline coronavirus (FCoV) RNA in the feces, tissues, and body fluids of infected cats. The RT-nPCR was targeted to the highly conserved 3'-untranslated region of the viral genome and will detect most, if not all, feline coronaviruses in the field. With the RT-nPCR FCoV RNA was detected in plasma samples from experimentally infected cats as early as 2 days postinoculation. FCoV RNA was also detected in serum, plasma, or ascitic fluid samples from 14 of 18 cats (78%) with naturally occurring feline infectious peritonitis (FIP). The use of RT-PCR for FLP diagnosis is limited because of the occurrence of apparently healthy FCoV carriers. These asymptomatic cats shed the virus in the feces and, in a number of cases, also had detectable virus in the plasma. Because of the nature of FCoV infections, our RT-PCR assay with plasma or serum cannot be used to establish a definite diagnosis of FIP. However, this assay does provide a new means to identify asymptomatic FCoV carriers. As such, RT-nPCR will be of use to screen cats before their introduction into FCoV-free catteries. Moreover, this assay provides ah important tool to study the epidemiology of FCoV.
引用
收藏
页码:684 / 689
页数:6
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