EXPRESSION OF PLASMA-MEMBRANE CA2+-PUMP EPITOPES IN HUMAN PLATELETS AND MEGAKARYOBLASTS

被引：10

作者：

CHENG, HY

MAGOCSI, M

COOPER, RS

PENNISTON, JT

BORKE, JL

机构：

[1] LOYOLA UNIV, SCH DENT, DEPT PHYSIOL PHARMACOL, 2160 S 1ST AVE, MAYWOOD, IL 60153 USA

[2] NATL INST HAEMATOL & BLOOD TRANSFUS, BUDAPEST, HUNGARY

[3] LOYOLA UNIV, STRITCH SCH MED, DEPT PREVENT MED & EPIDEMIOL, MAYWOOD, IL 60153 USA

[4] MAYO CLIN & MAYO FDN, DEPT BIOCHEM & MOLEC BIOL, ROCHESTER, MN USA

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 1994年 / 4卷 / 1-2期

关键词：

CA2+ PUMP; CA2+-MG2+ATPASE; PLATELET; MEGAKARYOBLAST; PCR; IMMUNOHISTOCHEMISTRY;

D O I：

10.1159/000154707

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human megakaryoblastic cells (platelet precursor cells) grown in culture were sectioned for immunohistochemistry. Incubating these cells with a monoclonal antibody (JA8) directed against the human erythrocyte Ca2+ pump showed specific staining of the plasma membranes. PCR analysis of RNA from these cells demonstrated specific sequences for type HPMCA1, HPMCA2 and HPMCA4 Ca2+ pump isoforms. Western blot analysis of megakaryoblastic cells, whole platelets, and platelet membranes using monoclonal antibodies JA8, 5F10, and 3C2, directed against plasma membrane Ca2+ pump showed staining of a major band of M(r) 120,000. Taken together, these studies demonstrate the existence of plasma membrane Ca2+ pump epitopes in human platelets and megakaryoblastic cells.

引用

页码：31 / 43

页数：13

共 46 条

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