A stable cytoplasmic male-sterile line of Brassica juncea carrying restructured organelle genomes from the somatic hybrid Trachystoma ballii+B-juncea

被引：27

作者：

Kirti, PB

Mohapatra, T

Baldev, A

Prakash, S

Chopra, VL

机构：

[1] National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi

来源：

PLANT BREEDING | 1995年 / 114卷 / 05期

关键词：

Brassica juncea; Trachystoma ballii; cytoplasmic male-sterility; chloroplast DNA recombination; mitochondrial DNA recombination; somatic hybrid;

D O I：

10.1111/j.1439-0523.1995.tb00826.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A cytoplasmic male-sterile (CMS) line of Brassica juncea has been developed by combining the cytoplasm originating from the somatic hybrid Trachystoma ballii+B. juncea, and the nucleus of B. juncea cv. Pusa Bold by repeated backcrossing. Male-sterile plants closely resembled the normal fertile B. juncea in general morphology, but had delayed flowering (5-7 days) when compared with fertile 'Pusa Bold' which flowered in 45 days. Stamens of the male-sterile line were transformed into petaloid structures. Pollen abortion occurred after tetrad formation. Female fertility of the male-sterile line was normal. Molecular analysis of organelle genomes indicated extensive mitochondrial DNA recombinations in the CMS line. Preliminary analysis of the chloroplast genome of the CMS line also indicated chloroplast DNA recombination.

引用

页码：434 / 438

页数：5

共 24 条

[1] NUCLEOTIDE-SEQUENCE OF THE CLUSTERED GENES FOR THE 44-KD CHLOROPHYLL-A APOPROTEIN AND THE 32KD-LIKE PROTEIN OF THE PHOTOSYSTEM-II REACTION CENTER IN THE SPINACH PLASTID CHROMOSOME [J].