SIMILARITY OF RESPONSES OF CULTURED SERTOLI CELLS TO CHOLERA TOXIN AND FSH

被引：55

作者：

FRITZ, IB ^{[1
]}

GRISWOLD, MD ^{[1
]}

LOUIS, BG ^{[1
]}

DORRINGTON, JH ^{[1
]}

机构：

[1] UNIV TORONTO, BANTING & BEST DEPT MED RES, TORONTO, ONTARIO, CANADA

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1976年 / 5卷 / 3-4期

关键词：

D O I：

10.1016/0303-7207(76)90090-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effects of cholera toxin [CT] on the responses of cultured Sertoli cells were compared with the effects of follicle-stimulating hormone (FSH) and N6O2''-dibutyryl-3'',5''-cyclic AMP (bu2cAMP). Addition of FSH or CT increased cAMP levels. Subsequently there were greater rates of conversion of testosterone to 17.beta.-estradiol, formation of androgen-binding protein (ABP) and incorporation of [3H]thymidine into DNA by Sertoli cells prepared from testes of immature rats and cultured in the presence of FSH or CT. Addition of bu2cAMP enhanced rates of formation of ABP, synthesis of 17.beta.-estradiol and synthesis of DNA. CT and bu2cAMP elicited changes in morphology of cultured Sertoli cells indistinguishable from those following FSH addition. Elevated intracellular cAMP levels can apparently duplicate known actions of FSH on cultured Sertoli cells; the possible obligatory role of cAMP in mediating FSH actions remains to be evaluated.

引用

页码：289 / 294

页数：6

共 18 条

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