A CORRELATION BETWEEN SOLUBLE BRAIN NITRIC-OXIDE SYNTHASE AND NADPH-DIAPHORASE ACTIVITY IS ONLY SEEN AFTER EXPOSURE OF THE TISSUE TO FIXATIVE

被引：412

作者：

MATSUMOTO, T

NAKANE, M

POLLOCK, JS

KUK, JE

FORSTERMANN, U

机构：

[1] NORTHWESTERN UNIV,SCH MED,DEPT PHARMACOL,CHICAGO,IL 60611

[2] ABBOTT LABS,ABBOTT PK,IL 60064

来源：

NEUROSCIENCE LETTERS | 1993年 / 155卷 / 01期

关键词：

NADPH-DIAPHORASE; NITRIC OXIDE SYNTHASE; CENTRAL NERVOUS SYSTEM; CYCLIC GMP; NITROBLUE TETRAZOLIUM;

D O I：

10.1016/0304-3940(93)90673-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In histochemical studies using fixed brain tissue, NADPH-diaphorase has been found to be colocalized with soluble nitric oxide synthase. In the present study, using fresh tissues from eight different regions of rat brain, NADPH-diaphorase activity was found mostly in the particulate fraction, whereas most of the nitric oxide synthase activity was located to the cytosolic fraction. Also, the distribution of NADPH-diaphorase activity among brain regions was different from that of nitric oxide synthase. Pretreatment of the fractions with paraformaldehyde virtually abolished the NADPH-diaphorase activity in the particulate fraction, whereas 40-60% of the NADPH-diaphorase activity remained intact in the cytosolic fraction. These results suggest that during fixation most NADPH-diaphorase activity is inactivated and only some of the NADPH-diaphorase activity associated with soluble nitric oxide synthase remains intact.

引用

页码：61 / 64

页数：4

共 16 条

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