ANTISENSE C-MYB OLIGONUCLEOTIDES INHIBIT INTIMAL ARTERIAL SMOOTH-MUSCLE CELL ACCUMULATION INVIVO

被引：679

作者：

SIMONS, M

EDELMAN, ER

DEKEYSER, JL

LANGER, R

ROSENBERG, RD

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

[2] MIT,DIV HLTH SCI TECHNOL,CAMBRIDGE,MA 02139

[3] MIT,DEPT CHEM ENGN,CAMBRIDGE,MA 02139

[4] HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,DEPT MED,BOSTON,MA 02215

来源：

NATURE | 1992年 / 359卷 / 6390期

关键词：

D O I：

10.1038/359067a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

SYNTHETIC antisense oligonucleotides have been used to dissect gene function in vitro. Technical difficulties prevented the use of this approach for investigating the effect of gene products in vivo. Here we report the use of local delivery of antisense c-myb oligonucleotide to suppress intimal accumulation of rat carotid arterial smooth muscle cells. Our results suggest that antisense oligonucleotides can be used to define the in vivo biological role of specific macromolecules in the blood vessel wall and could potentially serve as a new class of therapeutic agents for cardiovascular disorders.

引用

页码：67 / 70

页数：4

共 24 条

[1] PHARMACOKINETICS, BIODISTRIBUTION, AND STABILITY OF OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES IN MICE
AGRAWAL, S
TEMSAMANI, J
TANG, JY
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) : 7595 - 7599
[2] AN OLIGOMER COMPLEMENTARY TO C-MYB-ENCODED MESSENGER-RNA INHIBITS PROLIFERATION OF HUMAN MYELOID-LEUKEMIA CELL-LINES
ANFOSSI, G
GEWIRTZ, AM
CALABRETTA, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (09) : 3379 - 3383
[3] BROWN KE, 1992, J BIOL CHEM, V267, P4625
[4] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[5] CLOWES AW, 1983, LAB INVEST, V49, P327
[6] PLATELET-DERIVED GROWTH-FACTOR - STRUCTURE, FUNCTION, AND ROLES IN NORMAL AND TRANSFORMED-CELLS
DEUEL, TF
HUANG, JS
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1984, 74 (03) : 669 - 676
[7] A REVIEW OF THE PROLIFERATIVE BEHAVIOR, MORPHOLOGY AND PHENOTYPES OF VASCULAR SMOOTH-MUSCLE
DILLEY, RJ
MCGEACHIE, JK
PRENDERGAST, FJ
[J]. ATHEROSCLEROSIS, 1987, 63 (2-3) : 99 - 107
[8] EFFECT OF CONTROLLED ADVENTITIAL HEPARIN DELIVERY ON SMOOTH-MUSCLE CELL-PROLIFERATION FOLLOWING ENDOTHELIAL INJURY
EDELMAN, ER
ADAMS, DH
KARNOVSKY, MJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (10) : 3773 - 3777
[9] BASIC FIBROBLAST GROWTH-FACTOR ENHANCES THE COUPLING OF INTIMAL HYPERPLASIA AND PROLIFERATION OF VASA VASORUM IN INJURED RAT ARTERIES
EDELMAN, ER
NUGENT, MA
SMITH, LT
KARNOVSKY, MJ
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1992, 89 (02) : 465 - 473
[10] INHIBITION OF NEOINTIMAL SMOOTH-MUSCLE ACCUMULATION AFTER ANGIOPLASTY BY AN ANTIBODY TO PDGF
FERNS, GAA
RAINES, EW
SPRUGEL, KH
MOTANI, AS
REIDY, MA
ROSS, R
[J]. SCIENCE, 1991, 253 (5024) : 1129 - 1132

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