A NEW ESCHERICHIA-COLI HEAT-SHOCK GENE, HTRC, WHOSE PRODUCT IS ESSENTIAL FOR VIABILITY ONLY AT HIGH-TEMPERATURES

We identified and characterized a new Escherichia coli gene, htrC. Inactivation of the htrC gene results in the inability to form colonies at 42°C. An identical bacterial phenotype is found whether the htrC gene is inactivated either to Tn5 insertions or by a deletion spanning the entire gene. The htrC gene has been localized at 90 min, immediately downstream of the rpoC gene, and has been previously sequenced. It codes for a basic polypeptide with an M(r) of 21,130. The htrC gene is under heat shock regulation, since it is transcribed actively only in bacteria possessing functional σ32. Inactivation of htrC results in (i) bacterial filamentation at intermediate temperatures, (ii) cell lysis at temperatures above 42°C, (iii) overproduction of σ32-dependent heat shock proteins at all temperatures, (iv) overproduction of a few additional polypeptides, (v) underproduction of many polypeptides, and (vi) an overall defect in cellular proteolysis as judged by the reduced rate of puromycyl polypeptide degradation. In addition, the presence of an htrC mutation eliminates the UV sensitivity normally exhibited by lon mutant bacteria.