IDENTIFICATION AND CHARACTERIZATION OF A LEISHMANIA-DONOVANI ANTIGEN BELONGING TO THE 70-KDA HEAT-SHOCK PROTEIN FAMILY

被引：105

作者：

MACFARLANE, J ^{[1
]}

BLAXTER, ML ^{[1
]}

BISHOP, RP ^{[1
]}

MILES, MA ^{[1
]}

KELLY, JM ^{[1
]}

机构：

[1] UNIV LONDON LONDON SCH HYG & TROP MED, DEPT MED PARASITOL, KEPPEL ST, LONDON WC1E 7HT, ENGLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 190卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15586.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Leishmania donovani promastigote cDNA library was screened with serum obtained from a patient infected with visceral leishmaniasis. Sequence analysis of a clone obtained from this library revealed that the 600‐bp insert corresponded to the carboxy‐terminal region of an antigen related to the 70‐kDa heat‐shock protein family. The full‐length sequence of the corresponding gene (1959 nucleotides) was determined after isolation of genomic clones. Genes encoding the antigen are present on a single chromosome as a series of approximately twelve 3.7‐kb direct tandem repeats. The antigen can be identified as a 70‐kDa heat‐shock cognate protein by virtue of its molecular mass, sequence and constitutive expression during heat shock. It is expressed at all stages of the parasite life‐cycle. Antibodies against the λgt11 fusion protein were detected in more than 50% of serum samples obtained from patients with visceral leishmaniasis, but were not detected in sera from patients with cutaneous leishmaniasis or Chagas' disease. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：377 / 384

页数：8

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