SEROTONIN, BUT NEITHER NORADRENALINE NOR GABA, INHIBITS CAPSAICIN-EVOKED RELEASE OF IMMUNOREACTIVE SOMATOSTATIN FROM SLICES OF RAT SPINAL-CORD

It has been suggested that somatostatin is involved in nociceptive transmission in the dorsal horn and that it is contained in small primary afferent neurons. In the present experiments, to elucidate neural systems inhibiting the release of somatostatin from the primary afferent terminals, we examined the effects of serotonin, noradrenaline and gamma-aminobutyric acid on the capsaicin-evoked, dorsal-rhizotomy-sensitive and tetrodotoxin-insensitive release of immunoreactive somatostatin, 98.7% of which was somatostatin itself, from the dorsal-half slices of lumbar and cervical enlargements of rat spinal cord. Serotonin (30-100-mu-M) suppressed the evoked release in a concentration-dependent manner, and the suppression was antagonized by methysergide (100-mu-M). The evoked release of immunoreactive somatostatin was not inhibited by noradrenaline (100-mu-M) or gamma-aminobutyric acid (100-mu-M). The present results suggest that the serotonergic systems exert an inhibitory effect on the release of somatostatin from the central terminals of primary sensory neurons.