BLOCKING ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION OF ANTIBODIES TO ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPE-2

被引：43

作者：

NIELSEN, R ^{[1
]}

PLAMBECK, T ^{[1
]}

FOGED, NT ^{[1
]}

机构：

[1] ANIM BIOTECHNOL RES CTR,DK-1503 COPENHAGEN,DENMARK

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 04期

关键词：

D O I：

10.1128/JCM.29.4.794-797.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A blocking enzyme-linked immunosorbent assay (ELISA), based upon a polyclonal rabbit antiserum specific to Actinobacillus pleuropneumoniae serotype 2, was developed for the detection of antibodies to A. pleuropneumoniae serotype 2 in pigs. By testing sera from pigs experimentally infected with the 11 recognized serotypes of A. pleuropneumoniae, the assay was proven to be specific for A. pleuropneumoniae serotype 2. With field sera from herds infected with A. pleuropneumoniae serotype 2, the assay was found to be more sensitive than the complement fixation test. Positive results were not observed with field sera from herds known to be free from Actinobacillus infection or with sera from two herds infected with either A. pleuropneumoniae serotype 6 or 8. The high diagnostic sensitivity and specificity of the blocking ELISA will make it useful in field diagnostic work.

引用

页码：794 / 797

页数：4