THE APPLICATION OF EVANESCENT WAVE SENSING TO A HIGH-SENSITIVITY FLUOROIMMUNOASSAY

被引：27

作者：

WALCZAK, IM

LOVE, WF

COOK, TA

SLOVACEK, RE

机构：

[1] CIBA CORNING DIAGNOST CORP,RES & DEV,63 NORTH ST,MEDFIELD,MA 02052

[2] CORNING INC,CORNING,NY 14831

来源：

BIOSENSORS & BIOELECTRONICS | 1992年 / 7卷 / 01期

关键词：

FIBEROPTIC; IMMUNOSENSOR; FLUORESCENCE; EVANESCENT WAVE;

D O I：

10.1016/0956-5663(92)90029-M

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The principle of evanescent wave sensing was applied to immunological measurements of the clinically important human enzyme creatine kinase (CK; EC 2.7.3.2) isoenzyme MB form (CK-MB) with a molecular weight of about 84 000. The measurements were obtained using a green helium-neon laser based fluorimeter and the antibody conjugated fluorophor B-phycoerthrin (BPE). With conservative optical launch and collection angles of 25-degrees, a detection limit of 0.1 ng ml-1 CK-MB was observed in less than 15 min for a fiber optic based assay in which there were no subsequent washing, handling or processing steps after exposure to the sample.

引用

页码：39 / 48

页数：10

共 18 条

[1] REMOTE FIBER-OPTIC BIOSENSORS BASED ON EVANESCENT-EXCITED FLUORO-IMMUNOASSAY - CONCEPT AND PROGRESS [J].