ROUTINE SCREENING AND QUANTITATION OF URINARY CORTICOSTEROIDS USING BENCH-TOP GAS-CHROMATOGRAPHY MASS-SELECTIVE DETECTION

被引:32
作者
YAP, BK [1 ]
JOHNSTON, GAR [1 ]
KAZLAUSKAS, R [1 ]
机构
[1] UNIV SYDNEY,DEPT PHARMACOL,SYDNEY,NSW 2006,AUSTRALIA
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 573卷 / 02期
关键词
D O I
10.1016/0378-4347(92)80118-A
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method was developed for the routine screening, confirmation and quantitation of corticosteroids in human urine using bench top capillary gas chromatography (GC)-mass-selective detection. The free and conjugated corticosteroid fractions were isolated by liquid-liquid partition. After evaporation to dryness under vacuum the corticosteroid residues were derivatized to form the methyloxime trimethylsilyl ether derivatives. Both GC retention data and characteristic spectral data based on authentic reference standards were used for the identification and quantitation of cortisol, cortisone, tetrahydrocortisol and tetrahydrocortisone in the ppb (ng/ml) concentration range. The method is simpler and more efficient than the other GC-mass spectrometric (MS) techniques. It is also more sensitive than the liquid chromatographic-MS method.
引用
收藏
页码:183 / 190
页数:8
相关论文
共 26 条
[1]   2 CHROMATOGRAPHIC METHODS FOR THE DETERMINATION OF CORTICOSTEROIDS IN HUMAN BIOLOGICAL-FLUIDS - PHARMACOKINETIC APPLICATIONS [J].
ALHABET, SMH ;
ROGERS, HJ .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1989, 78 (08) :660-666
[2]  
BERTRAND PV, 1987, CLIN CHEM, V33, P2047
[3]   RIA VS CPB FOR PREDNISOLONE [J].
COLBURN, WA ;
BULLER, RH .
JOURNAL OF PHARMACOKINETICS AND BIOPHARMACEUTICS, 1975, 3 (05) :329-332
[4]   DERIVATIZATION AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC DETECTION OF ANABOLIC-STEROID RESIDUES ISOLATED FROM EDIBLE MUSCLE TISSUES [J].
DAESELEIRE, E ;
DEGUESQUIERE, A ;
VANPETEGHEM, C .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 562 (1-2) :673-679
[5]  
DAVIES CTM, 1973, J APPL PHYSIOL, V35, P887, DOI 10.1152/jappl.1973.35.6.887
[6]  
DEBOER D, 1990, COMMUNICATION
[7]  
DONIKE M, 1988, DOPE ANAL
[8]   CORTISOL PRODUCTION-RATES MEASURED BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY [J].
ESTEBAN, NV ;
YERGEY, AL .
STEROIDS, 1990, 55 (04) :152-158
[9]  
GASKELL SJ, 1983, CLIN CHEM, V29, P862
[10]  
GASKELL SJ, 1986, CLIN CHEM, V32, P536