PURIFICATION AND CHARACTERIZATION OF DIAMINE OXIDASE FROM ZEA-MAYS SHOOTS

被引：18

作者：

SUZUKI, Y

HAGIWARA, M

机构：

[1] Laboratory of Biological Science, Kyoritsu Women's College, Chiyoda-ku, Tokyo, 101, 2-2-1, Hitotsubashi

来源：

PHYTOCHEMISTRY | 1993年 / 33卷 / 05期

关键词：

ZEA-MAYS; GRAMINEAE; MAIZE; MAIZE SEEDLINGS; DIAMINE OXIDASE; ENZYME PURIFICATION;

D O I：

10.1016/0031-9422(93)85010-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diamine oxidase (DAO) (EC 1.4.3.6) activity was found in the shoots of seedlings of maize, oats, barley and wheat, using the quinoneimine reaction method. The enzyme was purified from maize shoots to apparent homogeneity using (NH4)2SO4 Precipitation and chromatography on DEAE cellulose, DEAE cellulose Sephadex A 50 and hydroxylapatite. The M(r) estimated by gel filtration was 11.8 x 10(4) and the SDS gel electrophoresis yielded a single band at M(r) 5.5 x 10(4). The K(m) value for putrescine was 25 x 10(-5) M. Maize DAO has a broad substrate specificity and is inhibited by sulphydryl reagents, copper binding agents and reagents reactive towards carbonyl radicals.

引用

页码：995 / 998

页数：4

共 13 条

[1] PURIFICATION AND CHARACTERIZATION OF DIAMINE OXIDASE FROM RICE EMBRYOS [J].