THE INHIBITORY EFFECTS OF INDOMETHACIN, NORDIHYDROGUAIARETIC ACID, AND PYRROLIDINEDITHIOCARBAMATE ON OVULATION AND PROSTAGLANDIN SYNTHESIS IN YELLOW PERCH (PERCA-FLAVESCENS) FOLLICLE INCUBATES

This study specifically determined the correlation between ovulation and ovarian prostaglandin F (PGF) and prostaglandin E (PGE) levels in yellow perch follicles treated with several eicosanoid synthesis inhibitors. in incubates treated with indomethacin (IM; .00001 to 10.0 mu g/ml) there was a significant dose-dependent correlation between ovulation and PGF/PGE levels, and between the two prostaglandins (PGs), further implicating PGs in the control of yellow perch ovulation. In incubates treated with nordihydroguaiaretic acid (NDGA), a traditional lipoxygenase inhibitor, there was a significant correlation between ovulation and PGF levels, yet there was no correlation between PGE levels and ovulation. A free-radical scavenger, pyrrolidinedithiocarbamate (PDTC) inhibited ovulation in a dose-dependent manner yet there was no correlation between either PGF or PGE levels and this inhibition, indicating that PDTC may block ovulation at other steps that involve free-radicals.