HYALURONAN RECEPTOR (CD44) EXPRESSION AND FUNCTION IN HUMAN PERIPHERAL-BLOOD MONOCYTES AND ALVEOLAR MACROPHAGES

被引:50
作者
CULTY, M [1 ]
OMARA, TE [1 ]
UNDERHILL, CB [1 ]
YEAGER, H [1 ]
SWARTZ, RP [1 ]
机构
[1] GEORGETOWN UNIV, MED CTR, DEPT PULM & CRIT CARE MED, WASHINGTON, DC 20007 USA
关键词
MACROPHAGE; MONOCYTE; CD44; RECEPTOR; HYALURONAN; HUMAN;
D O I
10.1002/jlb.56.5.605
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
CD44 glycoproteins are present on the surfaces of many hematopoietic cells and in some cases can bind hyaluronan, a major component of the extracellular matrix. In the present study, we have found that newly explanted human peripheral blood monocytes (PBMs) exhibit a major CD44 band of 85 kDa, whereas autologous alveolar macrophages (AM phi) express multiple isoforms ranging from 85 to 200 kDa. Within 4 h in culture, PBMs began expressing new CD44 isoforms of 120, 150, and 180 kDa. Newly explanted AM phi specifically bound [H-3]hyaluronan (135 cpm/mu g protein), but newly explanted PBMs did not. However, in vitro cultured PBM progressively acquired the ability to bind [H-3]hyaluronan and exhibited specific binding of hyaluronan similar to that of AM phi (113 cpm/mu g protein) after 4 days in culture. In both cases, the binding of [H-3]hyaluronan was specifically inhibited by the addition of monoclonal antibody directed against CD44. AM phi readily degraded [H-3]hyaluronan and reached a plateau after 4 days in culture (115 cpm/mu g protein). Newly explanted PBM exhibit no hyaluronan degradation and only a small deg radative activity after 4 days in culture (6 to 11 cpm/mu g protein). Thus, CD44 expression and function appear to change as PBM mature in vitro resembling more that found in AM phi.
引用
收藏
页码:605 / 611
页数:7
相关论文
共 36 条
[1]   EFFECTS OF ADHERENCE, ACTIVATION AND DISTINCT SERUM-PROTEINS ON THE INVITRO HUMAN MONOCYTE MATURATION PROCESS [J].
AKIYAMA, Y ;
GRIFFITH, R ;
MILLER, P ;
STEVENSON, GW ;
LUND, S ;
KANAPA, DJ ;
STEVENSON, HC .
JOURNAL OF LEUKOCYTE BIOLOGY, 1988, 43 (03) :224-231
[2]   EARLY CHANGES IN LUNG-TISSUE HYALURONAN (HYALURONIC-ACID) AND HYALURONIDASE IN BLEOMYCIN-INDUCED ALVEOLITIS IN HAMSTERS [J].
ANDERSON, B ;
SAMPSON, PM ;
OSMAN, M ;
GIANDOMENICO, A ;
TURINO, GM .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 143 (02) :284-288
[3]  
Bainton DF., 1992, INFLAMMATION BASIC P, V2nd ed., P303
[4]   THE HYALURONATE RECEPTOR IS A MEMBER OF THE CD44 (H-CAM) FAMILY OF CELL-SURFACE GLYCOPROTEINS [J].
CULTY, M ;
MIYAKE, K ;
KINCADE, PW ;
SILORSKI, E ;
BUTCHER, EC ;
UNDERHILL, C .
JOURNAL OF CELL BIOLOGY, 1990, 111 (06) :2765-2774
[5]   THE HYALURONAN RECEPTOR (CD44) PARTICIPATES IN THE UPTAKE AND DEGRADATION OF HYALURONAN [J].
CULTY, M ;
NGUYEN, HA ;
UNDERHILL, CB .
JOURNAL OF CELL BIOLOGY, 1992, 116 (04) :1055-1062
[6]   SURFACE MATRIX BINDING ALTERS MURINE PERITONEAL MONONUCLEAR PHAGOCYTE TNF-ALPHA AND IL-6 INDUCTION [J].
DARVILLE, T ;
TABOR, DR ;
THEUS, SA ;
JACOBS, RF .
IMMUNOLOGICAL INVESTIGATIONS, 1992, 21 (06) :539-552
[7]  
DENNING SM, 1990, J IMMUNOL, V144, P7
[8]  
GALINDO B, 1975, J RETICULOENDOTH SOC, V18, P295
[9]   AGGREGATION OF MACROPHAGES AND FIBROBLASTS IS INHIBITED BY A MONOCLONAL-ANTIBODY TO THE HYALURONATE RECEPTOR [J].
GREEN, SJ ;
TARONE, G ;
UNDERHILL, CB .
EXPERIMENTAL CELL RESEARCH, 1988, 178 (02) :224-232
[10]   EFFECT OF HYALURONIC-ACID ON PHAGOCYTOSIS OF OPSONIZED LATEX-PARTICLES [J].
HAKANSSON, L ;
HALLGREN, R ;
VENGE, P .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1980, 11 (06) :649-653