CHEMICAL-STRUCTURE OF NEUTRAL SUGAR CHAINS ISOLATED FROM HUMAN MATURE MILK KAPPA-CASEIN

被引：16

作者：

SAITO, T ^{[1
]}

ITOH, T ^{[1
]}

ADACHI, S ^{[1
]}

机构：

[1] TOHOKU UNIV, DEPT ANIM SCI, SENDAI, MIYAGI 980, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1988年 / 964卷 / 02期

关键词：

D O I：

10.1016/0304-4165(88)90169-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The carbohydrate chains linked to human .kappa.-casein from mature milk were released by alkaline borohydride treatment as reduced oligosaccharides. The neutral oligosaccharides of lower molecular weight were fractionated and purified by gel filtration and preparative thin layer chromatographies. Seven neutral oligosaccharides (a di- (0.5%), two tetra- (30.5%), two penta-(5.4%) and two hexasaccharide alditols (10.9%)) were obtained in homogeneity, and followed by methylation analysis with gas-liquid chromatography-mass spectrometry and by anomer analysis with 13C nuclear magnetic resonance. Their chemical structures were identified to be Gal.beta.1.fwdarw.3GalNAc-ol (I), Gal.beta.1.fwdarw.3[Gal.beta.1.fwdarw.4GlcNAc.beta.1.dag.AR6]GalNAc-ol (II), Gal.beta.1.fwdarw.[Fuc.alpha.1.fwdarw.4GlcNAc.beta.1.fwdarw.6]GalNAc-ol (III), GlcNAc.beta.1.fwdarw.3/6Gal.beta.1.fwdarw.3[Gal.beta.1-.fwdarw.4GlcNAc.beta.1.fwdarw.6]GalNAc-ol (IV), GlcNAc.beta.1.fwdarw.3/6Gal.beta.1.fwdarw.3[Fuc.alpha.1.fwdarw.4GlcNAc.beta.1.fwdarw.6]GalNAc-ol (V), Fuc.alpha.1.fwdarw.4GlcNAc.beta.1.fwdarw.3/6Gal.beta.1.fwdarw.3[Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.6]GalNAc-ol (VI) and Fuc.alpha.1.fwdarw.5GlcNAc.beta.1.fwdarw.3/6Gal.beta.1.fwdarw.3[Fuc.alpha.1.fwdarw.4GlcNAc.beta.1.fwdarw.6]GalNAc-ol (VII). Five oligosaccharide alditols (III-VII) were the novel carbohydrate chains of .kappa.-casein from mammalian milk.

引用

页码：213 / 220

页数：8

共 18 条

[1] BIFIDUS GROWTH-PROMOTING ACTIVITY OF A GLYCOMACROPEPTIDE DERIVED FROM HUMAN K-CASEIN [J].