METAL INHIBITION OF FERROCHELATASE ACTIVITY IN HUMAN-LYMPHOCYTES

被引：8

作者：

ROSSI, E ^{[1
]}

ATTWOOD, PV ^{[1
]}

GARCIAWEBB, P ^{[1
]}

COSTIN, KA ^{[1
]}

机构：

[1] UNIV WESTERN AUSTRALIA,DEPT BIOCHEM,NEDLANDS,AUSTRALIA

来源：

CLINICA CHIMICA ACTA | 1990年 / 188卷 / 01期

关键词：

Ferrochelatase; Kinetic analysis; Lymphocyte; Metal inhibition;

D O I：

10.1016/0009-8981(90)90141-E

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The activity of the terminal enzyme of haem biosynthesis, ferrochelatase (EC 4.99.1.1) was measured in sonicates of human lymphocytes. We used a sensitive method with zinc and mesoporphyrin as substrates and quantification of the product zinc-mesoporphyrin by HPLC. A variety of metal ions and organometal compounds were examined as possible inhibitors of ferrochelatase activity. Inhibition was observed with copper and mercury (but not with lead) and with tributyltin and methylmercury. The kinetics of ferrochelatase inhibition were examined for each of the four inhibitors identified. © 1990.

引用

页码：1 / 14

页数：14

共 19 条

[1] CHARACTERIZATION OF DEFICIENT HEME SYNTHASE ACTIVITY IN PROTOPORPHYRIA WITH CULTURED SKIN FIBROBLASTS [J].