BILIARY-EXCRETION OF FLUORESCENT CHOLEPHILES IN HEPATOCYTE COUPLETS - AN INVITRO MODEL FOR HEPATOBILIARY AND HEPATOTOXICITY STUDIES

被引:18
作者
FENTEM, JH
FOSTER, B
MILLS, CO
COLEMAN, R
CHIPMAN, JK
机构
[1] UNIV BIRMINGHAM,DEPT BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
[2] UNIV BIRMINGHAM,DEPT MED,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
关键词
D O I
10.1016/0887-2333(90)90099-F
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Hepatocyte couplets (26.8% of cell preparation with >85% viability) were prepared from rat livers. The preparation maintained the activity of 7-ethoxycoumarin-O-deethylase throughout an 8-hr incubation. Couplets extensively secreted the fluorescent cholephiles, fluorescein and cholyl-lysyl-fluorescein isothiocyanate, into sealed canalicular spaces within 2 hr and 15-30 min, respectively, which is indicative of functional tight junctions. The time scale for biliary secretion of the cholephiles correlated well with the relative kinetics of biliary excretion reported in vivo. Hepatocyte couplets may prove useful for studies on toxicity directed towards the hepatobiliary system. © 1990.
引用
收藏
页码:452 / 457
页数:6
相关论文
共 20 条
[1]  
Boyer J L, 1985, Trans Assoc Am Physicians, V98, P21
[2]   ENZYMATIC ASSAY FOR GLUTATHIONE [J].
BREHE, JE ;
BURCH, HB .
ANALYTICAL BIOCHEMISTRY, 1976, 74 (01) :189-197
[3]  
BRIDGES JW, 1981, TESTING TOXICITY, P125
[4]   BIOCHEMISTRY OF BILE SECRETION [J].
COLEMAN, R .
BIOCHEMICAL JOURNAL, 1987, 244 (02) :249-261
[5]   ACTIVITIES OF SEVERAL PHASE-I AND PHASE-II XENOBIOTIC BIOTRANSFORMATION ENZYMES IN CULTURED-HEPATOCYTES FROM MALE AND FEMALE RATS [J].
CROCI, T ;
WILLIAMS, GM .
BIOCHEMICAL PHARMACOLOGY, 1985, 34 (17) :3029-3035
[6]   ISOLATED RAT HEPATOCYTE COUPLETS IN SHORT-TERM CULTURE - STRUCTURAL CHARACTERISTICS AND PLASMA-MEMBRANE REORGANIZATION [J].
GAUTAM, A ;
NG, OC ;
BOYER, JL .
HEPATOLOGY, 1987, 7 (02) :216-223
[7]  
GEBHARDT R, 1982, J CELL SCI, V56, P233
[8]  
GEBHARDT R, 1986, RES ISOLATED CULTURE, P353
[9]   ISOLATED RAT HEPATOCYTE COUPLETS - A PRIMARY SECRETORY UNIT FOR ELECTROPHYSIOLOGIC STUDIES OF BILE SECRETORY FUNCTION [J].
GRAF, J ;
GAUTAM, A ;
BOYER, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (20) :6516-6520
[10]  
GUILLOUZO A, 1986, ISOLATED CULTURED HE, P313