CELLULAR-LOCALIZATION OF CYTOCHROME P450IIA1 IN TESTES OF MATURE SPRAGUE-DAWLEY RATS

被引：14

作者：

SENG, JE

LEAKEY, JE

ARLOTTO, MP

PARKINSON, A

GANDY, J

机构：

[1] UNIV ARKANSAS MED SCI HOSP, DEPT PHARMACOL & TOXICOL, 4301 W MARKHAM, SLOT 638, LITTLE ROCK, AR 72205 USA

[2] UNIV KANSAS, MED CTR, CTR ENVIRONM & OCCUPAT HLTH, KANSAS CITY, KS 66103 USA

[3] NATL CTR TOXICOL RES, JEFFERSON, AR 72079 USA

来源：

BIOLOGY OF REPRODUCTION | 1991年 / 45卷 / 06期

关键词：

D O I：

10.1095/biolreprod45.6.876

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Previous studies have shown that a prominent site of extrahepatic cytochrome P450IIA1 in male rats is the testis. We investigated the cellular location of cytochrome P450IIA1 in the testes of adult rats. Using specific isolation of testicular compartments and individual cell types, as well as in vivo removal of Leydig cells by ethane dimethyl sulfonate, we determined the cellular location of cytochrome P450IIA1 using testosterone hydroxylation assay, Western immunoblotting, and immunohistochemical analysis. Enriched Leydig cell fractions had the greatest testosterone 7-alpha-hydroxylase activity as well as immunoreactivity. Immunohistochemical analysis confirmed that the cellular location of cytochrome P450IIA1 was specific to Leydig cells. The specific localization of enzyme systems that are involved in xenobiotic activation may have important implications for inducing specific cell toxicity by compounds that exert their effects in the testes.

引用

页码：876 / 882

页数：7

共 36 条

[1] PURIFICATION OF 2 ISOZYMES OF RAT-LIVER MICROSOMAL CYTOCHROME-P450 WITH TESTOSTERONE 7-ALPHA-HYDROXYLASE ACTIVITY [J].