COLOCALIZATION OF F-ACTIN AND TALIN DURING FC RECEPTOR MEDIATED PHAGOCYTOSIS IN MOUSE MACROPHAGES

We have studied the distribution oftalin inJ774 cells and mouse peritoneal macrophages undergoing Fc receptor-mediated phagocytosis. At early stages ofphagocytosis, talin accumulates in the cells' cortical cytoplasm adjacent to the forming phagosome and extends into pseudopods that are encircling the particle. Talin colocalizes with F-actin at these sites. After particle ingestion is completed, F-actin and talin are no longer concentrated adjacent to phagosomes. Thus, talin and F-actin undergo dynamic and coordinate changes in their cytoplasmic location during Fc receptor-mediated phagocytosis. © 1990, Rockefeller University Press., All rights reserved.