IDENTIFICATION OF MONOCLONAL-ANTIBODIES CAPABLE OF DIFFERENTIATING ANTIGENIC VARIETIES OF EASTERN EQUINE ENCEPHALITIS VIRUSES

被引：50

作者：

ROEHRIG, JT ^{[1
]}

HUNT, AR ^{[1
]}

CHANG, GJ ^{[1
]}

SHEIK, B ^{[1
]}

BOLIN, RA ^{[1
]}

TSAI, TF ^{[1
]}

TRENT, DW ^{[1
]}

机构：

[1] NATL INST VIROL, Pune, INDIA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1990年 / 42卷 / 04期

关键词：

D O I：

10.4269/ajtmh.1990.42.394

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We have isolated and characterized 3 monoclonal antibody (Mab) reagents useful in the serological identification of varieties of eastern equine encephalitis (EEE) viruses. These antibodies were specific for the E1 glycoprotein of their homologous viruses. One Mab, 1B5C-3, reacted specifically with all North American (NA) EEE viruses isolated over a 50 year period. This antigenic stability of NA isolates was genetically confirmed by oligonucleotide fingerprinting. Evolutionary stability is a unique feature among alphaviruses. The Mab 1C1J-4 reacted specifically with 1 South American isolate of EEE virus. A third Mab, 1B1C-4, was EEE virus complex reactive. While none of these antibodies had virus neutralizing activity, the identified reactivities could be demonstrated in the more rapid serological tests of enzyme-linked immunosorbent assay and indirect immunofluorescence.

引用

页码：394 / 398

页数：5

共 15 条

[1] IDENTIFICATION OF 2 SOUTH AMERICAN STRAINS OF EASTERN EQUINE ENCEPHALOMYELITIS VIRUS FROM MIGRANT BIRDS CAPTURED ON MISSISSIPPI-DELTA [J].