IMPROVEMENT OF THE SANDWICH ELISA FOR THE DETERMINATION OF BASEMENT-MEMBRANE COLLAGEN COMPLEX IN SERA OF PATIENTS WITH CHRONIC LIVER-DISEASES BY UTILIZING THE CHAOTROPIC ION, NASCN

被引：2

作者：

AMANO, S

OHTA, U

FUJIWARA, K

MIYAKE, K

FUJII, S

YOSHIDA, T

MATSUBARA, O

MASUDA, Y

HAYASHI, T

机构：

[1] SHISEIDO RES CTR,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN

[2] SOCIAL HLTH INSURANCE MED CTR,DEPT INTERNAL MED,SHINJUKU KU,TOKYO 169,JAPAN

[3] SAITAMA MED SCH,DEPT INTERNAL MED 3,MOROYAMA,SAITAMA 35004,JAPAN

[4] TEIKYO UNIV,SCH MED,DEPT INTERNAL MED 1,ITABASHI KU,TOKYO 173,JAPAN

[5] TOKYO MED & DENT UNIV,SCH MED,DEPT PATHOL,BUNKYO KU,TOKYO 113,JAPAN

[6] UNIV TOKYO,COLL ARTS & SCI,DEPT LIFE SCI,MEGURO KU,TOKYO 153,JAPAN

来源：

INTERNATIONAL HEPATOLOGY COMMUNICATIONS | 1995年 / 4卷 / 01期

关键词：

SANDWICH ELISA; LIVER FIBROSIS; COLLAGEN; DIAGNOSIS OF CHRONIC LIVER DISEASES;

D O I：

10.1016/0928-4346(95)00204-V

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The sandwich ELISA for the determination of basement membrane collagen complex (BCC), which is composed of type IV collagen and basement membrane-associated collagen (BAC), in sera of patients with chronic liver diseases was improved by using the chaotropic ion, NaSCN. NaSCN activated the immunoreactivity of endogenous BCC in sera in a concentration-dependent manner and showed the maximum effect at 400 mM. Intra- andinter-assay coefficients of variation were below 6.9% and below 11.9%, respectively. Recovery of standard BCC added to a normal or a pathological serum was 102.4 to 108.0%. The BCC levels in sera of patients with chronic hepatitis and liver cirrhosis showed significantly higher values than those in serum of healthy volunteers, which was consistent with the immunohistochemical analysis in which the staining of BAC of the liver cirrhotic tissue was much increased as compared with that of normal liver tissue.

引用

页码：1 / 8

页数：8

共 18 条

[1]

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