ANTIBIOTIC-SENSITIVITY TESTING OF BACTERIA BY MICROCOLONY INHIBITION AND IMAGE-ANALYSIS

被引：5

作者：

HAMMONDS, SJ

ADENWALA, F

机构：

[1] Department of Life Sciences, Trent Ploytechnic, Nottingham

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1990年 / 10卷 / 01期

关键词：

Image analyzers - Image-analysis - Microcolonies - Microscope slide - Rapid method - Staphylococcus aureus;

D O I：

10.1111/j.1472-765X.1990.tb00087.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A rapid method for determining the sensitivity of Staphylococcus aureus to β‐lactarns is described. The method involves the culture of microcolonies of the bacterium on the surface of antibiotic‐containing agar medium on microscope slides. The areas of the microcolonies formed are measured by means of a microscope and image analyzer. The sensitivity of the bacterium can be estimated in 4 h by this technique and the results are comparable to those obtained by overnight incubation. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：27 / 29

页数：3