CELL-SPECIFIC AND INDUCER-SPECIFIC ACCRETION OF HUMAN ISOMETALLOTHIONEINS

被引：25

作者：

CAVIGELLI, M ^{[1
]}

KAGI, JHR ^{[1
]}

HUNZIKER, PE ^{[1
]}

机构：

[1] UNIV ZURICH, INST BIOCHEM, WINTERTHURERSTR 190, CH-8057 ZURICH, SWITZERLAND

来源：

BIOCHEMICAL JOURNAL | 1993年 / 292卷

关键词：

D O I：

10.1042/bj2920551

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The synthesis of metallothionein (MT) was investigated in three different human epithelial cell lines, each derived from one of the embryonic germ layers. The accretion of different isoforms of the protein was monitored using a sensitive neutral-pH h.p.l.c. method. Induction of MT synthesis by zinc ions and dexamethasone revealed differences between the three cell lines, both with respect to the number and the amounts of the different isoMTs formed. Dose-response experiments showed that an increase in dexamethasone concentration enhances MT accretion asymptotically to a limit, whereas within the concentration range explored zinc produces an exponential augmentation.

引用

页码：551 / 554

页数：4

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