CHARACTERIZATION OF PEA CHLOROPLASTIC CARBONIC-ANHYDRASE - EXPRESSION IN ESCHERICHIA-COLI AND SITE-DIRECTED MUTAGENESIS

被引：31

作者：

PROVART, NJ ^{[1
]}

MAJEAU, N ^{[1
]}

COLEMAN, JR ^{[1
]}

机构：

[1] UNIV TORONTO, DEPT BOT, 25 WILLCOCKS ST, TORONTO M5S 3B2, ON, CANADA

来源：

PLANT MOLECULAR BIOLOGY | 1993年 / 22卷 / 06期

关键词：

CARBONIC ANHYDRASE; ESCHERICHIA-COLI; MUTAGENESIS; PISUM-SATIVUM;

D O I：

10.1007/BF00028967

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding the mature, chloroplast-localized carbonic anhydrase in pea has been expressed in E. coli. The enzyme is fully active and yields of up to 20% of the total soluble protein can be obtained from the bacteria. This expression system was used to monitor the effects of site-directed mutagenesis of seven residues found within conserved regions in the pea carbonic anhydrase amino acid sequence. The effects of these modifications are discussed with respect to the potential of various amino acids to act as sites for zinc coordination or intramolecular proton shuttles.

引用

页码：937 / 943

页数：7

共 21 条

[1] PLANT CARBONIC ANHYDRASES .1. DISTRIBUTION OF TYPES AMONG SPECIES [J].

ATKINS, CA ;

PATTERSON, BD ;

GRAHAM, D .

PLANT PHYSIOLOGY, 1972, 50 (02) :214-+

[2] THE ROLE OF THE METAL-ION IN THE REFOLDING OF DENATURED BOVINE CO(II)-CARBONIC ANHYDRASE-II [J].

BERGENHEM, N ;

CARLSSON, U .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 998 (03) :277-285

[3] SPINACH CHLOROPLASTIC CARBONIC-ANHYDRASE - NUCLEOTIDE-SEQUENCE ANALYSIS OF CDNA [J].

BURNELL, JN ;

GIBBS, MJ ;

MASON, JG .

PLANT PHYSIOLOGY, 1990, 92 (01) :37-40

[4]

CHRISTIANSON DW, 1991, ADV PROTEIN CHEM, V42, P281

[5] REFINED STRUCTURE OF HUMAN CARBONIC ANHYDRASE-II AT 2.0-A RESOLUTION [J].

ERIKSSON, AE ;

JONES, TA ;

LILJAS, A .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1988, 4 (04) :274-282

[6]

FAWCETT TW, 1990, J BIOL CHEM, V265, P5414

[7] A GENE HOMOLOGOUS TO CHLOROPLAST CARBONIC-ANHYDRASE (ICFA) IS ESSENTIAL TO PHOTOSYNTHETIC CARBON-DIOXIDE FIXATION BY SYNECHOCOCCUS PCC7942 [J].

FUKUZAWA, H ;

SUZUKI, E ;

KOMUKAI, Y ;

MIYACHI, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4437-4441

[8]

KUNKEL TA, 1987, METHOD ENZYMOL, V154, P367

[9] ISOLATION AND CHARACTERIZATION OF A CDNA CODING FOR PEA CHLOROPLASTIC CARBONIC-ANHYDRASE [J].

MAJEAU, N ;

COLEMAN, JR .

PLANT PHYSIOLOGY, 1991, 95 (01) :264-268

[10] NUCLEOTIDE-SEQUENCE OF A COMPLEMENTARY-DNA ENCODING TOBACCO CHLOROPLASTIC CARBONIC-ANHYDRASE [J].

MAJEAU, N ;

COLEMAN, JR .

PLANT PHYSIOLOGY, 1992, 100 (02) :1077-1078

← 1 2 3 →