EVIDENCE FOR A STRONG PATERNAL EFFECT ON HUMAN PREIMPLANTATION EMBRYO DEVELOPMENT AND BLASTOCYST FORMATION

被引:227
作者
JANNY, L [1 ]
MENEZO, YJR [1 ]
机构
[1] FDN MERIEUX,IRH,BRON,FRANCE
关键词
HUMAN BLASTOCYST FORMATION; FROZEN SPERM; ABNORMAL SPERM; HUMAN IVF;
D O I
10.1002/mrd.1080380107
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In human in vitro fertilization (I.V.F.), it was first assumed that all the embryos obtained had the same developmental potential whatever the quality of sperm. However, this has not been confirmed. We have used the coculture technique and determined the blastocyst formation rate in three groups of patients: group 1: patients with normal sperm count(> 20 x 10(6)/ml), motility (> 30%), and morphology (> 50%); group 2: patients treated by I.V.F. with frozen donor sperm; group 3: patients with severely impaired sperm quality (< 3 x 10(6) forward motile and morphologically normal spermatozoa per mi). In group 1, we found a strong correlation between cleavage rate and blastocyst formation rate (P < 0.0001) with a blastocyst formation rate comprised between 40% and 50%. This was not true for the two other groups for which the overall number of blastocysts obtained and the number of patients having at least one blastocyst were severely reduced (P < 0.0001). These data are discussed in terms of DNA quality, timing of formation of the pronuclei, and delays in cell cycles at the time of genomic activation. These observations lead to a new approach to the study of fertilizing ability of poor quality sperm. It may help in the decision as to whether couples treated for male infertility should be excluded from I.V.F. protocols. (C) 1994 Wiley-Liss, Inc.
引用
收藏
页码:36 / 42
页数:7
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