THE MOST ABUNDANT NASCENT POLY(A)+ RNAS ARE TRANSCRIBED BY RNA POLYMERASE-III IN MURINE TUMOR-CELLS

被引:27
作者
KRAMEROV, DA
TILLIB, SV
SHUMYATSKY, GP
GEORGIEV, GP
机构
[1] Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow B-334
关键词
D O I
10.1093/nar/18.15.4499
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Twelve to twenty percent of newly synthesized poly(A) + RNA is transcribed by RNA polymerase III in Ehrlich ascites carcinoma and P3O1 plasmocytoma mouse tumors. Most of this RNA designated as pol lllpoly(A) + RNA has a size of 160 to 800 nucleotides with a maximum of distribution of ca. 300 nucleotides. Pol lllpoly(A) + RNA fraction consists of two major classes of molecules corresponding to previously described B1 RNA and B2 RNA with the ratio of 1:4 to 2:3. All B2 RNAs present in poly(A)+ fraction contain a long poly(A) segments at the 3′ ends. Thus, RNA polymerase III transcripts can be polyadenylated. Several transcripts that hybridize with B2 probe were also observed in poly(A)-RNA. The major components consist of 180, 160, 120 and 95 nucleotides. The 180-nucleotide B2 RNA seems to be a primary transcript from B2 repeat. We suggest that other B2 RNAs are transcribed from truncated copies of B2 element. © 1990 Oxford University Press.
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页码:4499 / 4506
页数:8
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