ALTERED GLUCOSE TRANSPORTER MESSENGER-RNA ABUNDANCE IN A RAT MODEL OF ENDOTOXIC-SHOCK

被引：36

作者：

ZELLER, WP

THE, SM

SWEET, M

GOTO, M

GOTTSCHALK, ME

HURLEY, RM

FILKINS, JP

HOFMANN, C

机构：

[1] LOYOLA UNIV, STRITCH SCH MED, DEPT PHYSIOL, MAYWOOD, IL 60153 USA

[2] LOYOLA UNIV, STRITCH SCH MED, INST SHOCK & TRAUMA, MAYWOOD, IL 60153 USA

[3] LOYOLA UNIV, STRITCH SCH MED, RES SERV, MAYWOOD, IL 60153 USA

[4] LOYOLA UNIV, STRITCH SCH MED, DEPT BIOCHEM, MAYWOOD, IL 60153 USA

[5] LOYOLA UNIV, STRITCH SCH MED, DEPT SURG, MAYWOOD, IL 60153 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90958-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To better understand molecular mechanisms of glucose transport in shock, we studied glucose transporter isoform mRNA abundance after injection of S. enteritidis endotoxin (40mg/kg) or saline. Six to 8 hours after injection, endotoxin-treated animals compared to controls became hypoglycemic (44±6 vs. 111±4 mg/dl) and lactacidemic (5.9±0.5 vs. 1.3±0.1). At such times, tissue RNA was isolated and hybridized to Riboprobes for GLUT1 (erythrocyte), GLUT2 (liver), and GLUT4 (muscle/fat) glucose transporter isoforms and expressed as percent of control. GLUT1 mRNA abundance was increased in fat (660%, p<.05), soleus muscle (314%, p<.05), and liver (871%, p<.001) of endotoxin-treated rats. Soleus muscle GLUT4 mRNA levels were increased (+33%, p<.02), while liver GLUT2 mRNA levels were markedly decreased (-58%, p<.01). The overall increase in GLUT1 mRNA abundance accompanied by lowered liver GLUT2 mRNA levels may either cause or reflect profoundly altered glucose transport. © 1991.

引用

页码：535 / 540

页数：6

共 29 条

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