RECENT DEVELOPMENTS IN QUANTITATIVE AFFINITY-CHROMATOGRAPHY

被引：14

作者：

WINZOR, DJ

机构：

[1] Department of Biochemistry, University of Queensland, Brisbane

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 597卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80097-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review surveys developments during the past decade in the use of quantitative affinity chromatography as a means of evaluating equilibrium constants for solute-ligand and solute matrix interactions. Topics include allowance for multivalency of the partitioning solute, removal of the myth that highly substituted affinity matrices are unsuitable for zonal quantitative affinity chromatography, adaptation of the technique to allow characterization of high-affinity interactions and the application of quantitative affinity chromatography theory to the characterization of biospecific adsorption phenomena in cellular systems.

引用

页码：67 / 82

页数：16

共 67 条

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