GENETIC-ANALYSIS OF HOMOMERIC INTERACTIONS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE USING THE YEAST 2-HYBRID SYSTEM

被引：57

作者：

KALPANA, GV

GOFF, SP

机构：

[1] Biochemistry/Molec. Biophysics Dept., Columbia Univ. Coll. of Phys./Surg., New York

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 22期

关键词：

RETROVIRAL REPLICATION; INTEGRATION; PROTEIN PROTEIN INTERACTIONS; GAL4;

D O I：

10.1073/pnas.90.22.10593

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The retroviral integrase protein (IN) is responsible for catalyzing a concerted integration reaction in which the two termini of linear viral DNA are joined to host DNA. To probe the potential for IN to form protein multimers, we used the yeast two-hybrid system. The coexpression of a GAL4 DNA binding domain-IN fusion and a GAL4 activation domain-IN fusion together resulted in the successful activation of a GAL4-responsive LacZ reporter gene. The system was used to examine a variety of IN deletion mutants. The results suggest that the central region of the protein is necessary for multimerization and that the N-terminal zinc finger region is not important.

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页码：10593 / 10597

页数：5

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