FETUS-SPECIFIC EXPRESSION OF A FORM OF CYTOCHROME-P-450 IN HUMAN LIVERS

被引:162
作者
KOMORI, M
NISHIO, K
KITADA, M
SHIRAMATSU, K
MUROYA, K
SOMA, M
NAGASHIMA, K
KAMATAKI, T
机构
[1] HOKKAIDO UNIV,FAC MED,DEPT PATHOL,SAPPORO,HOKKAIDO 060,JAPAN
[2] SAPPORO MED COLL,DEPT SURG,SAPPORO,HOKKAIDO 060,JAPAN
[3] SAPPORO MED COLL,DEPT PATHOL,SAPPORO,HOKKAIDO 060,JAPAN
[4] MOCHIDA PHARMACEUT CO LTD,KITA KU,TOKYO 115,JAPAN
关键词
D O I
10.1021/bi00470a024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The developmentally regulated expression of forms of cytochrome P-450, namely, those encoded by λHFL33 and NF25 or HLp cDNAs, which were isolated from respective fetal and adult human liver cDNA libraries, was investigated. When EcoRI fragments of cDNA clones of λHFL33 and NF25 were used as probes, these probes hybridized with RNA from both fetal and adult human livers. However, when oligonucleotides specific to the coding and ′-noncoding region of λHFL33 (oli-HFL and oli-HFL3′, respectively) were used as probes, these probes gave hybridizable bands with RNA from fetal but not adult livers. On the other hand, an oligonucleotide probe specific to the coding region of NF25 and HLp (oli-NF) gave positive bands with RNA only from adult livers. These results indicate that P-450(HFL33) is expressed specifically in fetal livers and that neither P-450NF nor HLp is expressed in fetal livers, but one or both are expressed in adult livers. © 1990, American Chemical Society. All rights reserved.
引用
收藏
页码:4430 / 4433
页数:4
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