ISOLATION OF EMS-INDUCED MUTANTS IN ARABIDOPSIS ALTERED IN SEED FATTY-ACID COMPOSITION

被引：141

作者：

JAMES, DW

DOONER, HK

机构：

[1] DNA Plant Technology, Oakland, 94608, CA

来源：

THEORETICAL AND APPLIED GENETICS | 1990年 / 80卷 / 02期

关键词：

Arabidopsis thaliana; Fatty acid desaturation; Lipid biosynthesis; Oilseed breeding; Seed lipids;

D O I：

10.1007/BF00224393

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Mutants of Arabidopsis thaliana were identified by screening pedigreed M3 seed collections from EMS-treated plants for changes in fatty acid (FA) composition. The FA phenotypes of the most dramatic mutants are as follows: G30 and 1E5 (allelic) lack linolenic acid (18:3) and are elevated in linoleic acid (18:2); 4A5 is deficient in 18:2 and 18:3 and fourfold increased in oleic acid (18:1); 9A1 lacks all FAs > C18 and is twofold increased in 18:1; 1A9 is twofold increased in palmitic acid (16:0) and decreased by one-half in 18:1; 2A11 is two-to threefold increased in stearic acid (18:0) and decreased by one-half in 18:1. Based on segregation of F2 selfed plants derived from crosses to wild type, all of these phenotypes are the result of single gene mutations. © 1990 Springer-Verlag.

引用

页码：241 / 245

页数：5

共 19 条

[1] FATTY-ACID COMPOSITION OF LEAF LIPIDS DETERMINED AFTER COMBINED DIGESTION AND FATTY-ACID METHYL-ESTER FORMATION FROM FRESH TISSUE [J].