HYPEREXPRESSION AND PURIFICATION OF ESCHERICHIA-COLI ADENYLATE-CYCLASE USING A VECTOR DESIGNED FOR EXPRESSION OF LETHAL GENE-PRODUCTS

被引：96

作者：

REDDY, P

PETERKOFSKY, A

MCKENNEY, K

机构：

[1] NINCDS,MOLEC GENET LAB,BETHESDA,MD 20892

[2] NHLBI,BIOCHEM GENET LAB,BETHESDA,MD 20205

来源：

NUCLEIC ACIDS RESEARCH | 1989年 / 17卷 / 24期

关键词：

D O I：

10.1093/nar/17.24.10473

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：10473 / 10488

页数：16

共 42 条

[1] THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE ADENYLATE-CYCLASE GENE OF ESCHERICHIA-COLI [J].

AIBA, H ;

MORI, K ;

TANAKA, M ;

OOI, T ;

ROY, A ;

DANCHIN, A .

NUCLEIC ACIDS RESEARCH, 1984, 12 (24) :9427-9440

[2]

AIBA H, 1985, J BIOL CHEM, V260, P3063

[3]

Bernard H U, 1979, Methods Enzymol, V68, P482

[4] BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION [J].

BIGGIN, MD ;

GIBSON, TJ ;

HONG, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :3963-3965

[5]

BIRNBOIM HC, 1983, METHOD ENZYMOL, V100, P243

[6] GENETIC CHARACTERIZATION OF MUTATIONS WHICH AFFECT CATABOLITE-SENSITIVE OPERONS IN ESCHERICHIA-COLI, INCLUDING DELETIONS OF GENE FOR ADENYL-CYCLASE [J].

BRICKMAN, E ;

SOLL, L ;

BECKWITH, J .

JOURNAL OF BACTERIOLOGY, 1973, 116 (02) :582-587

[7] IMPROVED OLIGONUCLEOTIDE SITE-DIRECTED MUTAGENESIS USING M13 VECTORS [J].

CARTER, P ;

BEDOUELLE, H ;

WINTER, G .

NUCLEIC ACIDS RESEARCH, 1985, 13 (12) :4431-4443

[8] IDENTIFICATION OF THE ESCHERICHIA-COLI CYA GENE-PRODUCT AS AUTHENTIC ADENYLATE-CYCLASE [J].

DANCHIN, A ;

GUISO, N ;

ROY, A ;

ULLMANN, A .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 175 (03) :403-408

[9] CONTROL MECHANISM OF BACTERIAL GROWTH BY CYCLIC ADENOSINE 3'-MONOPHOSPHATE 5' - MONOPHOSPHATE [J].

DEROBERTIS, EM ;

JUDEWICZ, ND ;

TORRES, HN .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 55 (03) :758-764

[10] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

← 1 2 3 4 5 →