OXIDATIVE STRESS AND TUMOR-CELL PROLIFERATION

被引:138
作者
BURDON, RH [1 ]
GILL, V [1 ]
RICEEVANS, C [1 ]
机构
[1] ROYAL FREE HOSP,SCH MED,DEPT BIOCHEM,LONDON NW3 2PF,ENGLAND
来源
FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 11卷 / 1-3期
关键词
Active oxygen species; Cell proliferation; Hydrogen peroxide; Paraquat; Transformed cells; αtocopherol;
D O I
10.3109/10715769009109669
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of oxidant stress were studied in immortalised hamster (BHK-21) and rat (208F) cell lines before and after transformation to the malignant state with polyoma virus, or activated H-ras, respectively. Whilst intracellular superoxide production was detectable in both transformed and immortalised cells the rate was somewhat higher in the transformed cells which have lower levels of superoxide dismutase. Because growth of transformed cells was particularly depressed in the presence of MTT, a tetrazolium compound reduced by superoxide, the possible role of active oxygen species in the promotion of cell growth was examined. Low levels of hydrogen peroxide were stimulatory towards both immortalised and transformed cells. In the case of H-ras transformed rat cells, paraquat was also stimulatory provided serum was present in the growth medium. In the absence of serum, paraquat was notably inhibitory but inhibition could be alleviated by addition of low concentrations of αtocopherol (10-8 M) to the serum-depleted medium. Although depletion of serum from the growth medium also leads to lower cell proliferation, subsequent experiments showed that a-tocopherol addition to serum-free medium was sufficient to restimulate growth. In the case of transformed cells, yields of cells were even greater than that encountered in the presence of 10% serum. Thus whilst certain active oxygen species (e. g. hydrogen peroxide) may have a role in promoting the growth of transformed and immortalised cells the necessity for antioxidant protection is important. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
引用
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页码:65 / 76
页数:12
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