THE OPPOSITE AND ANTAGONISTIC EFFECTS OF THE CLOSELY-RELATED POU FAMILY TRANSCRIPTION FACTORS BRN-3A AND BRN-3B ON THE ACTIVITY OF A TARGET PROMOTER ARE DEPENDENT ON DIFFERENCES IN THE POU DOMAIN

被引：74

作者：

MORRIS, PJ

THEIL, T

RING, CJA

LILLYCROP, KA

MOROY, T

LATCHMAN, DS

机构：

[1] UCL, SCH MED, DEPT MOLEC PATHOL, MED MOLEC BIOL UNIT, LONDON W1P 6DB, ENGLAND

[2] UNIV MARBURG, INST MOLEK BIOL & TUMORFORSCH, D-35037 MARBURG, GERMANY

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1994年 / 14卷 / 10期

关键词：

D O I：

10.1128/MCB.14.10.6907

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Brn-3a, Brn-3b, and Brn-3c POU family transcription factors are closely related to one another and are members of the group IV subfamily of POU factors. Here we show that despite this close relationship, the factors have different effects on the activity of a target promoter: Brn-3a and Brn-3c stimulate the promoter whereas Brn-3b represses it. Moreover, Brn-3b can antagonize the stimulatory effect of Brn-3a on promoter activity and can also inhibit promoter activation by the Oct-2.1 POU factor. The difference in the transactivation activities of Brn-3a and Brn-3b is dependent upon the C-terminal region containing the POU domain of the two proteins, since exchange of this domain between the two factors converts Brn-3a into a repressor and Brn-3b into an activator.

引用

收藏

页码：6907 / 6914

页数：8

相关论文

共 41 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] THE DNA TARGET SITE FOR THE BRN-3 POU FAMILY TRANSCRIPTION FACTORS CAN CONFER RESPONSIVENESS TO CYCLIC-AMP AND REMOVAL OF SERUM IN NEURONAL CELLS [J].

BUDHRAMMAHADEO, V ;

THEIL, T ;

MORRIS, PJ ;

LILLYCROP, KA ;

MOROY, T ;

LATCHMAN, DS .

NUCLEIC ACIDS RESEARCH, 1994, 22 (15) :3092-3098

[3] THE ENHANCER OF HUMAN PAPILLOMAVIRUS TYPE-16 - BINDING-SITES FOR THE UBIQUITOUS TRANSCRIPTION FACTOR-OCT-1, FACTOR-NFA, FACTOR-TEF-2, FACTOR-NF1, AND FACTOR-AP-1 PARTICIPATE IN EPITHELIAL CELL-SPECIFIC TRANSCRIPTION [J].

CHONG, T ;

APT, D ;

GLOSS, B ;

ISA, M ;

BERNARD, HU .

JOURNAL OF VIROLOGY, 1991, 65 (11) :5933-5943

[4] A NOVEL POU HOMEODOMAIN GENE SPECIFICALLY EXPRESSED IN CELLS OF THE DEVELOPING MAMMALIAN NERVOUS-SYSTEM [J].

COLLUM, RG ;

FISHER, PE ;

DATTA, M ;

MELLIS, S ;

THIELE, C ;

HUEBNER, K ;

CROCE, CM ;

ISRAEL, MA ;

THEIL, T ;

MOROY, T ;

DEPINHO, R ;

ALT, FW .

NUCLEIC ACIDS RESEARCH, 1992, 20 (18) :4919-4925

[5] TRANSCRIPTIONAL REGULATION OF THE HUMAN PAPILLOMAVIRUS-16 E6-E7 PROMOTER BY A KERATINOCYTE-DEPENDENT ENHANCER, AND BY VIRAL E2 TRANSACTIVATOR AND REPRESSOR GENE-PRODUCTS - IMPLICATIONS FOR CERVICAL CARCINOGENESIS [J].

CRIPE, TP ;

HAUGEN, TH ;

TURK, JP ;

TABATABAI, F ;

SCHMID, PG ;

DURST, M ;

GISSMANN, L ;

ROMAN, A ;

TUREK, LP .

EMBO JOURNAL, 1987, 6 (12) :3745-3753

[6] THE CONSTITUTIVELY EXPRESSED OCTAMER BINDING-PROTEIN OTF-1 AND A NOVEL OCTAMER BINDING-PROTEIN EXPRESSED SPECIFICALLY IN CERVICAL CELLS BIND TO AN OCTAMER-RELATED SEQUENCE IN THE HUMAN PAPILLOMAVIRUS-16 ENHANCER [J].

DENT, CL ;

MCINDOE, GAJ ;

LATCHMAN, DS .

NUCLEIC ACIDS RESEARCH, 1991, 19 (16) :4531-4535

[7] THE B-CELL AND NEURONAL FORMS OF THE OCTAMER-BINDING PROTEIN OCT-2 DIFFER IN DNA-BINDING SPECIFICITY AND FUNCTIONAL-ACTIVITY [J].

DENT, CL ;

LILLYCROP, KA ;

ESTRIDGE, JK ;

THOMAS, NSB ;

LATCHMAN, DS .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (08) :3925-3930

[8] A GENETIC PATHWAY FOR THE DEVELOPMENT OF THE CAENORHABDITIS-ELEGANS HSN MOTOR NEURONS [J].

DESAI, C ;

GARRIGA, G ;

MCINTIRE, SL ;

HORVITZ, HR .

NATURE, 1988, 336 (6200) :638-646

[9] BRN-3.0 - A POU-DOMAIN PROTEIN EXPRESSED IN THE SENSORY, IMMUNE, AND ENDOCRINE SYSTEMS THAT FUNCTIONS ON ELEMENTS DISTINCT FROM KNOWN OCTAMER MOTIFS [J].

GERRERO, MR ;

MCEVILLY, RJ ;

TURNER, E ;

LIN, CR ;

OCONNELL, S ;

JENNE, KJ ;

HOBBS, MV ;

ROSENFELD, MG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (22) :10841-10845

[10]

Gorman C., 1985, DNA CLONING PRACTICA, V1, P143

← 1 2 3 4 5 →