ACCUMULATED GLUTAMATE LEVELS IN THE SYNAPTIC VESICLE ARE NOT MAINTAINED IN THE ABSENCE OF ACTIVE-TRANSPORT

被引：27

作者：

CARLSON, MD

UEDA, T

机构：

[1] UNIV WISCONSIN,MENTAL HLTH RES INST,205 WASHTENAW PL,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT PHARMACOL,MADISON,WI 53706

[3] UNIV WISCONSIN,DEPT PSYCHIAT,MADISON,WI 53706

来源：

NEUROSCIENCE LETTERS | 1990年 / 110卷 / 03期

关键词：

ATP-dependent uptake; Electrochemical proton gradient; Glutamate translocator; Steady-state glutamate level; Synaptic vesicle;

D O I：

10.1016/0304-3940(90)90868-A

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have investigated factors which may affect accumulated glutamate levels in synaptic vesicles and glutamate efflux. Agents which dissipate the electrochemical proton gradient resulted in a rapid reduction of steady-state vesicular glutamate levels, which was prevented by N-ethylmaleimide. Glutamate efflux was found to occur even in the presence of an electrochemical proton gradient, but was effectively inhibited by N-ethylmaleimide. These results suggest that accumulated glutamate levels in synaptic vesicles are not maintained unless glutamate is taken up continuously by an active transport mechanism, and they could provide an explanation for the lack of convincing evidence for the enrichment of endogenous glutamate in isolated synaptic vesicles. © 1990.

引用

页码：325 / 330

页数：6

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